For this reason, the normalized MFEs strongly decrease as hyperbolic functions of length and produce unreliable results when applied for the comparison of sequences with different sizes. Here, we demonstrate that the variation of MFE with sequence length is not linear and is significantly biased by the mathematical formula used for the normalization procedure. Although this normalization procedure has been used in several studies, the relationship between normalized MFE and length has not yet been investigated in detail.
Simple indices, obtained by dividing the MFE by the number of nucleotides, have been used for a direct comparison of the folding stability of RNAs of various sizes.
The minimum free energy (MFE) of ribonucleic acids (RNAs) increases at an apparent linear rate with sequence length.
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